ABSTRACT
Aim To establish the model of H2O2-induced damage in retinal pigment epithelial cells,and to investigate the protective effects of blueberry anthocyanin.Methods Cell viability was determined by MTT assay and the reactive oxygen species (ROS) level was measured using fluorescence assay.We selected H2O2 solution of different concentrations from 0 to 3 200 μmol · L-1 to stimulate ARPE-19 cells for 2,6 and 24 h,and compared the effects of blueberry anthocyanins of 1,5 and 10 mg · L-1 on cells pretreated with 6 h and 24 h.The model of oxidative damage was then established and applied in the next study,which focused on the protective effects of major constituents in blueberry anthocyanins,malvidin and its glycosides.Results Model condition of H2O2-induced damage in ARPE-19 cells was 800 μmol · L-1 H2O2 for 2 h.The cell viability decreased to 63.69％ when stimulated by H2O2.With pretreatment of 5 mg · L-1 blueberry anthocyanin extract (BAE),pure anthocyanin malvidin (My) and its glucoside (Mv-3-glc) and galactoside (Mv-3-gal),ARPE-19 cell viabilities were significantly improved to 86.57％,115.72％,98.15％ and 127.97％,respectively (P ＜ 0.01).Meanwhile,the ROS levels were attenuated by 52.38％,95.38％,77.55％ and 116.09％,respectively (P ＜ 0.05).Conclusion Blueberry anthocyanin could significantly inhibit the oxidative damage induced by H2O2 in retinal pigment epithelial cells,and malvidin and its glycosides contribute to the protective effects.